一家动物研究机构的三只七岁非洲爪蛙（Xenopus laevis）因体重减轻、体腔膨胀和游泳步态异常而被出于福利原因安乐死。每只动物的完整尸检显示，两只动物的左肝叶有局灶性、坚硬、深棕色至绿色肿块，伴有多灶性出血，另一只动物则有透壁、坚硬、米色、多叶状胃肿块。此外，其中一只患有肝脏肿块的青蛙患有血腔瘤。组织学上，肝脏肿块被诊断为肝细胞腺瘤，胃肿块被诊断为胃癌并有跨体腔转移。使用以下抗体对这三个肿瘤进行免疫表型分析：细胞角蛋白 AE1/AE3、波形蛋白、E-钙粘蛋白、P53 和 Ki67，此外，仅针对肝肿瘤，使用突触素、Prox-1、S100 和 Sox-9。在选定的病例中还进行了马森三色、高碘酸希夫染色和革兰氏染色。其中一个肝细胞腺瘤被 E-钙粘蛋白弱阳性标记，而另一个肝细胞腺瘤仅显示 Sox-9 的可变阳性。波形蛋白标记基质和窦内皮细胞。有趣的是，在肝脏中，细胞角蛋白 AE1/AE3 标记仅限于胆管上皮和肝窦内皮。胃癌仅用细胞角蛋白AE1/AE3标记为阳性。本报告旨在指导实验动物兽医准确诊断两栖动物的多器官肿块。据作者所知，这是第一个针对非洲爪蟾肝细胞和胃肿瘤病例系列的全面形态学研究。

Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.