非洲爪蛙（Xenopus laevis）中自发的肝细胞和胃肿瘤
Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis)
影响因子:1.30000
分区:农林科学4区 / 兽医学4区 动物学4区
发表日期:2025 Apr
作者:
Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet
摘要
来自动物研究机构的三个七岁的非洲爪蛙（Xenopus laevis）显示体重减轻，卵形延伸和异常游泳步态在福利地面上安乐死。每只动物的完整尸检显示，左肝叶中有两只动物的焦点，深褐色至绿色的肿块，并在另一种动物的两只动物中有多灶性出血和一只透壁，坚固，米色，多叶的胃肿块。此外，其中一只肝肿块的青蛙患有血肿。从组织学上讲，肝肿块被诊断为肝细胞腺瘤和胃肿块，是具有反式旋转型转移酶的胃癌。使用以下抗体对这三个肿瘤进行免疫型：细胞角蛋白AE1/AE3，波形蛋白，E-钙粘蛋白，p53和Ki67，此外，仅肝肿瘤，仅肝肿瘤，突触突触蛋白，Prox-1，S100和Sox-9。在某些情况下，还进行了Masson的三色，周期性酸Schiff和Gram染色。肝细胞腺瘤之一用e-钙粘着蛋白弱标记，而另一个仅显示SOX-9的阳性。波形蛋白标记了基质和正弦内皮。有趣的是，在肝脏中，细胞角蛋白AE1/AE3标记仅限于胆道上皮和正弦内皮。胃癌仅用细胞角蛋白AE1/AE3积极标记。该报告旨在指导实验室兽医准确诊断两栖动物的多器官群众。据作者所知，这是关于X. laevis中的一系列肝细胞和胃肿瘤病例系列的首次全面形态学研究。
Abstract
Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.