非洲爪蛙（Xenopus laevis）自发性肝细胞及胃肿瘤

Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis)

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LABORATORY ANIMALS

影响因子:1.3

分区:农林科学4区 / 兽医学4区动物学4区

发表日期:2025 Apr

作者: Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres, Alejandro Suárez-Bonnet

DOI: 10.1177/00236772241273060

摘要

来自动物研究设施的三只七岁非洲爪蛙（Xenopus laevis）表现出体重减轻、腹腔膨胀和异常游泳步态，出于动物福利考虑被安乐死。每只动物的完整尸检显示，两只动物的左肝叶出现局灶性坚实、深棕色至绿色的肿块伴多发出血，另一只动物则发现穿壁性坚实、米色、多叶的胃肿块。此外，患有肝肿块的其中一只青蛙还伴有血腔肿瘤。在组织学上，肝肿块被诊断为肝细胞腺瘤，胃肿块为胃癌伴跨腔转移。三种肿瘤使用以下抗体进行免疫表型分析：细胞角蛋白AE1/AE3、波形蛋白（vimentin）、E-钙黏蛋白（E-cadherin）、P53和Ki67，此外，仅对肝肿瘤进行的检测还包括突触素（synaptophysin）、Prox-1、S100和Sox-9。在部分病例中还进行了马恩氏三色染色、周期酸希夫（PAS）染色和革兰氏染色。一例肝细胞腺瘤对E-cadherin显示弱阳性标记，另一例仅对Sox-9显示变异性阳性。波形蛋白标记基质和窦状内皮细胞。值得注意的是，肝脏中细胞角蛋白AE1/AE3的标记仅限于胆管上皮和窦状内皮。胃癌仅对细胞角蛋白AE1/AE3呈阳性。本报告旨在指导实验室动物兽医准确诊断两栖动物多器官肿块。据作者所知，这是首次对X. laevis中肝细胞和胃部新生物的病例系列进行全面形态学研究。

Abstract

Three seven-year-old African clawed frogs (Xenopus laevis) from an animal research facility showed weight loss, coelomic distention and an abnormal swimming gait were euthanised on welfare grounds. A complete necropsy of each animal showed a focal, firm, dark brown to green mass with multifocal haemorrhages in the left liver lobe in two animals and a transmural, firm, beige, multilobulated gastric mass in another animal. Additionally, one of the frogs with the hepatic mass had haemocoeloma. Histologically, the hepatic masses were diagnosed as hepatocellular adenomas and the gastric mass as a gastric carcinoma with trans-coelomic metastases. The three tumours were immunophenotyped using the following antibodies: cytokeratin AE1/AE3, vimentin, E-cadherin, P53 and Ki67 and, additionally, for the hepatic tumours only, synaptophysin, Prox-1, S100 and Sox-9. Masson's trichrome, Periodic acid Schiff and Gram stains was also performed in selected cases. One of the hepatocellular adenomas was weakly positively labelled with E-cadherin whereas the other showed variable positivity for Sox-9 only. Vimentin labelled the stroma and sinusoidal endothelia. Interestingly in the liver, the cytokeratin AE1/AE3 labelling was restricted to the biliary epithelium and sinusoidal endothelia. The gastric carcinoma labelled positively with cytokeratin AE1/AE3 only. This report aims to guide laboratory animal veterinarians to accurately diagnose multi-organ masses in amphibians. To the authors' knowledge, this is the first comprehensive morphological study on a case series of hepatocellular and gastric neoplasia in X. laevis.