牙龈卟啉单胞菌（P.gingivalis）是一种与多种胃肠道癌症相关的重要牙周病原体。然而，牙龈卟啉单胞菌是否可以促进口腔鳞状细胞癌（OSCC）以及与这种促进相关的潜在机制仍不清楚。在本研究中，使用OSCC异种移植模型来评估牙龈卟啉单胞菌对肿瘤进展的影响。功能研究是在体外对几种 OSCC 细胞系进行的。使用牙龈卟啉单胞菌特异性 16S rRNA 荧光原位杂交 (FISH) 来测试其在临床样本中的患病率。我们发现牙龈卟啉单胞菌增加了 OSCC 裸模型中的肿瘤体积和肿瘤生长。功能研究表明，牙龈卟啉单胞菌通过促进细胞自噬来抑制 OSCC 细胞的凋亡。口腔鳞癌患者的 FISH 样本中牙龈卟啉单胞菌比白斑患者或健康受试者的 FISH 样本中更为常见（分别为 70% vs. 47.2% vs. 33.3%，p = 0.045 和 p < 0.001）。 gingivalis 在 OSCC 进展中发挥加速作用，并通过增强自噬途径减少癌细胞凋亡来促进 OSCC。© 2024 Wiley periodicals LLC。

Porphyromonas gingivalis (P. gingivalis) is a keystone periodontal pathogen associated with various gastro-intestinal tract cancers. However, whether P. gingivalis can promote oral squamous cell carcinoma (OSCC) and the underlying mechanism associated with such promotion remain unclear.In this study, OSCC xenograft models were used to evaluate the effects of P. gingivalis on tumor progression. The functional studies were done on several OSCC cell lines in vitro. P. gingivalis-specific 16S rRNA fluorescent in situ hybridization (FISH) was used to test its prevalence in clinical samples.We found that P. gingivalis increased tumor volume and tumor growth in OSCC nude models. Functional studies demonstrated that P. gingivalis inhibited the apoptosis of OSCC cells by promoting cellular autophagy. P. gingivalis was more prevalent in FISH samples from patients with OSCC than from patients with leukoplakia or healthy subjects (70% vs. 47.2% vs. 33.3%, p = 0.045 and p < 0.001, respectively).These data suggest that P. gingivalis plays an accelerating role in OSCC progression and contributes to OSCC by enhancing the autophagy pathway to reduce carcinoma apoptosis.© 2024 Wiley Periodicals LLC.