赖氨酸 5 上的组蛋白 H2A (H2AacK5) 和赖氨酸 27 上的 H3 (H3AcK27) 乙酰化通过 p300 酶在病理病变中调节多种细胞机制；然而，它们在牙源性病变中的作用尚未得到解决。本研究旨在评估成釉细胞瘤（AMB）（n = 30）、牙源性角化囊肿（OK）（n = 15）、腺瘤样牙源性肿瘤（AOT）（n = 10）样本中p300、H2AacK5和H3AcK27的免疫表达，牙源性纤维瘤 (OF) (n = 8)、钙化性牙源性囊肿 (COC) (n = 8)、牙源性粘液瘤 (MIX) (n = 10) 和成釉细胞纤维瘤 (AF) (n = 06)。 AOT 中 p300 阳性细胞的百分比较高，COC、OK、AMB、AF、OF 和 MIX 中 p300 阳性细胞百分比下降。 H2AacK5 阳性细胞在 AF 中较高，在 AOT、COC、OK、OF、AMB 和 MIX 中减少，而 H3acK27 阳性细胞在 AOT 中较高，在 COC、OK、AF、OF、AMB 和 MIX 中减少。与侵袭性病变相比，这些蛋白质在非侵袭性病变中的表达更高。 AMB、MIX 和 OF 中 p300 与 H2AacK5 和 H3acK27 呈正相关，而 AOT 和 COC 中 p300 与 H2AacK5 呈正相关。组蛋白乙酰化可能与这些病变的生物学行为有关，可用于改善其诊断和治疗。© 2024 John Wiley

The acetylation of histones H2A on lysine 5 (H2AacK5) and H3 on lysine 27 (H3AcK27) modulate several cellular mechanisms through the p300 enzyme in pathological lesions; however, their role in odontogenic lesions has not been addressed. This study aims to evaluate the immunoexpression of p300, H2AacK5, and H3AcK27 in samples of ameloblastoma (AMB) (n = 30), odontogenic keratocyst (OK) (n = 15), adenomatoid odontogenic tumor (AOT) (n = 10), odontogenic fibroma (OF) (n = 8), calcifying odontogenic cyst (COC) (n = 8), odontogenic myxoma (MIX) (n = 10), and ameloblastic fibroma (AF) (n = 06). The percentage of p300-positive cells was higher in AOT and decreased in COC, OK, AMB, AF, OF, and MIX. H2AacK5-positive cells were higher in AF and decreased in AOT, COC, OK, OF, AMB, and MIX, whereas H3acK27-positive cells were higher in AOT and decreased in COC, OK, AF, OF, AMB, and MIX. The expression of these proteins was higher in nonaggressive lesions in comparison to aggressive lesions. There was a positive correlation between p300 and H2AacK5, and H3acK27 in AMB, MIX, and OF, whereas there was a positive correlation between p300 and H2AacK5 in AOT and COC. The histone acetylation may be involved in the biological behavior of these lesions, which could be used to improve their diagnosis and treatment.© 2024 John Wiley & Sons A/S.